Mono Sex Culture:Mono-sex culture is based on the culture of fish by producing all males or all females depending upon
the sex
which have better food conversion ratio and growth rate. Sex of fish
genetically is determined
by the sex
chromosomes (X, Y, Z, or W). The male determining gene M is present on any of
the three X,
Y and W. in
XY mechanism, the females are XX and males are XO. Some species have ZZ female
and ZW
male. In
platy fish there are 3 sex chromosomes – X, Y and W; XX, WX and WY are some
combinations.
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However for
all male or female productions the flowing procedures are maintained –
The sex of
fish is identified before maturity and male and females are separated. The
process is
laborious.
Desired quantity of male or females are not produced by that process.
Experimental
hybridization in Tilapia can produce monosex stock. Inter specific and intra
specific
mating
yields monosex male stocks as follows
♂ T, macrohir × ♀ T.
nilotica
♂ T. hornorum × ♀ T.
mossambica
♂ T. mossambica × ♀ T.
nilotica
♂ T. mossambica (African) × ♀ T.
mossambica (Malaysian)
Treatment
with sex hormones: It is another easiest way, when male sex hormone methyl
testosterone
is
administered through feeding in early developmental stages of female fish. The
genotype female
(XX) then
transferred to phenotype male (XX). If such sex reversed male are crossed with
normal
female, the
progeny will be 100% female. The gonads of fish (teleost) are undifferentiated
at early
stages of
maturity and it can be triggered to produce male or female gonads by that
process.
Sex reversed
male (XX) × Normal female (XX)
↓
Female (XX)
……. F1
The hormone
treated sex reversed male are generally not fit for human consumption. But F1
progeny is
normal
female and suitable for human consumption. But sometimes the culturist does not
produce F1
progeny to
produce only male population. In case of Tilapia the little amount of methyl
testosterone
(15-60mg/kg
of food) is administered. The drug is given for 30-50 days of life, during which
gonadal
differentiation
takes place. The uses of estrogenic steroids are not successful. However the
production
of all
females has been attempted in salmon and trout. Oral administration of 17-ß
estradiol at
20mg/kg of
food is given to the juvenile trout & salmon up to 60 days resulted sex
reversal of males to
females. In
pacific salmon (Onchorhynchus spp.), the immersion of young fish in drug as
well as feeding
appears to
be necessary for sex reversal.
Necessity of
Monosex Culture:
Some time
one sex of certain species has better growth rate and food conversion
efficiency. To culture
that sex
(male/female) monosex culture is essential. For example we can say that the
male Tilapia
grows faster
than female, then the culture of male is beneficial in case Tilapia.
When the
fecundity of certain species is very much high and if they can breed in
captivity without any
inducing
agent, there is the possibility of overcrowding of fish, which leads to stunted
growth (due to
the prolific
reproduction e.g. Tilapia).
The
production of monosex fish is easier than the production of sterile fish, so in
commercial purpose
generally
monosex cultured is mostly prefer.
Example of
Monosex Production in Nature:
According to
recent report, the flat fish population in the estuary of British River Tyne, a
heavily
polluted
river, was found more than 50% of males possessing abnormal testes. The
tendency of their
sex change
from male to female was probably due to the female hormones. But the actual
reason and
mechanism of
the action is not known.
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